Latest News from the Welsher Lab

The return of the CRS!

After a long two years, the annual Chemistry Research Symposium (CRS) returned to Duke Chemistry! It was a great day of talks organized by the graduate students. Shawn, Stacey, and Courtney all gave fantastic talks on their recent works in the world of real-time microscopy!

Interested in rotations?

Welcome to the new cohort of graduate students to Duke Chemistry!

Are you interested in capturing biology and chemistry at speeds and scales previously impossible? Want to learn more about the initial contacts of viruses with host tissue or analyze the behavior of single molecules in complex environments? Like to build things or work with your hands? Like to code or want to learn how to code? If any of these fit you, come do a rotation with us! If you want more information, just contact Kevin directly.

We are currently accepting students to our single-virus (more info: Mapping the Early Stages of Viral Infection) and single-molecule tracking projects (more info: Real-time 3D Microscopy).

The secret in situ life of the protein corona

Xiaochen has developed a “lock on” spectroscopy for measuring the protein corona on freely diffusing single nanoparticles in situ. The data showed that the “full corona”, comprised of the tightly bound “hard corona” and weakly bound “soft corona”, contains twice as many proteins as ex situ measured hard corona. Therefore, when nanoparticles are removed from the biological fluid for analysis, about half of the proteins that are present in situ are lost. This method shows how critical it is the characterize the nano-bio interface in the native biological solution!

This work has been selected as a “Hot Paper” and will be featured on the back inside cover of an upcoming issue of Angewandte Chemie Int. Ed.. Way to go, Xiaochen! #hotpapersummer

Link to paper: https://onlinelibrary.wiley.com/doi/10.1002/anie.202105741

Cover link: https://onlinelibrary.wiley.com/doi/10.1002/anie.202107758

Choose your photons carefully

In a newly published paper, Chen examines the “information-efficiency” of different scanning patterns for 3D single-particle tracking. Using Fisher information as a guide, Chen demonstrates that localization precision can be improved nearly two-fold by selectively sampling high-information off-center locations. Check out the full paper here:

https://doi.org/10.3390/e23050498

This paper is included in the special issue on Recent Advances in Single-Particle Tracking: Experiment and Analysis in Entropy.

Congratulations to Dr. Xiaoqi Lang!!!

Xiaoqi successfully defended her thesis entitled “Uncovering the ‘Shape’ of Intracellular Water by Hyperspectral Stimulated Raman Scattering Microscopy”. Congratulations to Dr. Lang, the first PhD from our group! Her work established a new line of inquiry in the lab that will persist for years to come. We wish Xiaoqi all the best as she moves on to her postdoctoral work with Prof. Wei Min at Columbia University!

Welcome Jonathan!

Jonathan Aguilar joins the single-virus tracking / 3D-TrIm team. Jonathan follows a proud tradition of exceptional Duke undergrads diving into the wonderful world of active microscopy. Welcome aboard, Jonathan!

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