Publications

Preprints

  1. Lin, Y.; Exell, J.; Lin, H., Zhang, C.; Welsher, K.D. Hour-long, kilohertz sampling rate 3D single-virus tracking in live cells enabled by StayGold fluorescent protein fusions. bioRxiv (2024) doi:10.1101/2024.03.14.585070
  2. Hou, S.; Zhang, C.; Niver, A.; Welsher, K. D., Mapping nanoscale forces and potentials in live cells with microsecond 3D single-particle tracking. bioRxiv, (2022). doi:10.1101/2022.06.27.497788

Publications

  1. Tan, X.; Hou. S.; Niver, A.; Zhang, C.; Johnson, A.; Welsher. K.D., Active-Feedback 3D Single-Molecule Tracking Using a Fast-Responding Galvo Scanning Mirror. The Journal of Physical Chemistry A 127, 6320-6328 (2023).  https://pubs.acs.org/doi/10.1021/acs.jpca.3c02090 Preprint:doi:10.1101/2023.03.28.534562
  2. Niver, A. J.; Welsher, K. D. Using COBWEBS for 3D active-feedback tracking of freely diffusing particles in tricky environments. Proc.SPIE 12386, 1238605, (2023).
  3. Johnson, C.;* Exell, J.;* Lin, Y.; Aguilar, J.; Welsher, K. D., Capturing the start point of the virus-cell interaction with high-speed 3D single-virus tracking. Nat. Methods 19, 1642-1652, (2022). (bioRxiv preprint) Featured on Duke Today and Biomedical Picture of the Day (MRC LMS)! See also the News & Views on the “Next generation single virus tracking” and the research highlight in Nature!

  4. Niver, A.; Welsher, K.D., Combined online Bayesian and windowed estimation of background and signal localization facilitates active-feedback particle tracking in complex environments. J. Chem. Phys. 157, 184108 (2022). (bioRxiv preprint) 
  5. Kim, J.; Jozic, A.; Lin, Y.; Eygeris, Y.; Bloom, E.; Tan, X., Acosta, C.; MacDonald, K. D.; Welsher, K. D.; Sahay, G., Engineering Lipid Nanoparticles for Enhanced Intracellular Delivery of mRNA through Inhalation. ACS Nano 16, 14792-14806 (2022).
  6. Yu, D.; Garcia, A.; Blum, S. A.; Welsher, K. D., Growth Kinetics of Single Polymer Particles in Solution via Active-Feedback 3D Tracking. Journal of the American Chemical Society 144, 14698–14705, (2022).(ChemRxiv preprint)jacsat_v144i032-2image
  7. Tan, X. & Welsher, K. Particle-by-Particle in Situ Characterization of the Protein Corona via Real-Time 3D Single Particle Tracking Microscopy. Angewandte Chemie International Edition 60, 22359-22367 (2021). (chemrxiv). Featured on Inside Back Cover!
  8. Zhang, C. & Welsher, K. Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy. Entropy 23, 498 (2021). Special Issue – “Recent Advances in Single-Particle Tracking: Experiments and Analysis.” (biorxiv) Featured on cover! Click here for the Video Abstract courtesy of encyclopedia.pub!
  9. Hou, S., Exell, J. & Welsher, K. Real-time 3D single molecule tracking. Nature Communications 11, 3607 (2020).
  10. Lang, X. & Welsher, K. Mapping solvation heterogeneity in live cells by hyperspectral stimulated Raman scattering microscopy. The Journal of Chemical Physics 152, 174201 (2020). (Emerging Investigators Collection) (chemrxiv).
  11. Patel, S., Ashwanikumar, N., Robinson, E., Xia, Y., Mihai, C., Griffith, J. P., Hou, S., Esposito, A. A., Ketova, T., Welsher, K., Joyal, J. L., Almarsson, Ö. & Sahay, G. Naturally-occurring cholesterol analogues in lipid nanoparticles induce polymorphic shape and enhance intracellular delivery of mRNA. Nature Communications 11, 983 (2020).
  12. Johnson, C., Exell, J., Kuo, J. & Welsher, K. Continuous focal translation enhances rate of point-scan volumetric microscopy. Opt. Express 27, 36241-36258 (2019) arXiv:1909.12893.
  13. Hou, S. & Welsher, K. An Adaptive Real-Time 3D Single Particle Tracking Method for Monitoring Viral First Contacts. Small 15, 1903039 (2019).
  14. Hou, S., Johnson, C. & Welsher, K. Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells. Molecules 24, 2826 (2019). Special Issue – “Single Molecule Fluorescence”
  15. Hou, S., Welsher, K. A Protocol for Real-time 3D Single Particle Tracking. J. Vis. Exp. (131), e56711, doi:10.3791/56711 (2018).

  16. Hou, S., Lang, X. & Welsher, K. Robust Real-time 3D Single Particle Tracking Using a Dynamically Moving Laser Spot. Opt. Lett. 42, 2390-2393, (2017). Featured on the Duke Research Blog and Techcrunch!

Graduate and Postdoctoral Publications

  1. Li, W.-Y.; Yin, S.; Huang, S.-W.; Yang, M.-H.; Chen, P. MT.; Wu, S.-R.; Welsher, K.; Yang, H.; Chen; A. Y.-M. The Trajectory Patterns of Single HIV-1 Virus-like Particle in Live CD4 Cells: A Real Time Three-dimensional Multi-resolution Microscopy Study Using Encapsulated Nonblinking Giant Quantum Dot. Journal of Microbiology, Immunology and Infection. (2022).
  2. Welsher, K. & Yang, H. Imaging the behavior of molecules in biological systems: Breaking the 3D speed barrier with 3D Multi-resolution Microscopy. Faraday Discuss. 108, 359-379, (2015). Cover Story!c5fd00090d copy
  3. Welsher, K., McManus, S. A., Hsia, C.-H., Yin, S. & Yang, H. Discovery of Protein- and DNA-Imperceptible Nanoparticle Hard Coating Using Gel-Based Reaction Tuning. J. Am. Chem. Soc. 137, 580-583, (2015).141103-TOC-01
  4. Welsher, K. & Yang, H. Multi-resolution 3D visualization of the early stages of cellular uptake of peptide-coated nanoparticles. Nat. Nanotechnol. 9, 198-203, (2014).140925-landing-only-01
  5. Chen, Z., Hong, G., Wang, H., Welsher, K., Tabakman, S. M., Sherlock, S. P., Robinson, J. T., Liang, Y. & Dai, H. Graphite-Coated Magnetic Nanoparticle Microarray for Few-Cells Enrichment and Detection. ACS Nano 6, 1094-1101, (2012).
  6. Welsher, K. & Yang, H. Model-Free Analysis of Time-Dependent Single-Molecule Spectroscopy Dynamics of Biological Macromolecules. 2012 9th IEEE International Symposium on Biomedical Imaging (ISBI), 921-923, (2012).
  7. Welsher, K., Sherlock, S. P. & Dai, H. J. Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared window. Proc. Natl. Acad. Sci. U. S. A. 108, 8943-8948, (2011).
  8. Hong, G. S., Tabakman, S. M., Welsher, K., Chen, Z., Robinson, J. T., Wang, H. L., Zhang, B. & Dai, H. J. Near-Infrared-Fluorescence-Enhanced Molecular Imaging of Live Cells on Gold Substrates. Angew. Chem., Int. Ed. 50, 4644-4648, (2011).
  9. Tabakman, S. M., Welsher, K., Hong, G. S. & Dai, H. J. Optical Properties of Single-Walled Carbon Nanotubes Separated in a Density Gradient: Length, Bundling, and Aromatic Stacking Effects. J. Phys. Chem. C 114, 19569-19575, (2010).
  10. Robinson, J. T., Welsher, K., Tabakman, S. M., Sherlock, S. P., Wang, H. L., Luong, R. & Dai, H. J. High Performance In Vivo Near-IR (> 1 µm) Imaging and Photothermal Cancer Therapy with Carbon Nanotubes. Nano Research 3, 779-793, (2010).
  11. Hong, G. S., Tabakman, S. M., Welsher, K., Wang, H. L., Wang, X. R. & Dai, H. J. Metal-Enhanced Fluorescence of Carbon Nanotubes. J. Am. Chem. Soc. 132, 15920-15923, (2010).
  12. Zhang, L., Tu, X. M., Welsher, K., Wang, X. R., Zheng, M. & Dai, H. J. Optical Characterizations and Electronic Devices of Nearly Pure (10,5) Single-Walled Carbon Nanotubes. J. Am. Chem. Soc. 131, 2454-2455, (2009).
  13. Welsher, K., Liu, Z., Sherlock, S. P., Robinson, J. T., Chen, Z., Daranciang, D. & Dai, H. A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice. Nat. Nanotechnol. 4, 773-780, (2009).
  14. Prencipe, G., Tabakman, S. M., Welsher, K., Liu, Z., Goodwin, A. P., Zhang, L., Henry, J. & Dai, H. PEG Branched Polymer for Functionalization of Nanomaterials with Ultralong Blood Circulation. J. Am. Chem. Soc. 131, 4783-4787, (2009).
  15. Liu, Z., Tabakman, S. M., Welsher, K. & Dai, H. Carbon nanotubes in biology and medicine: In vitro and in vivo detection, imaging and drug delivery. Nano Research 2, 85-120, (2009).
  16. Welsher, K., Liu, Z., Daranciang, D. & Dai, H. Selective Probing and Imaging of Cells with Single Walled Carbon Nanotubes as Near-Infrared Fluorescent Molecules. Nano Lett. 8, 586-590, (2008).
  17. Sun, X., Zaric, S., Daranciang, D., Welsher, K., Lu, Y., Li, X. & Dai, H. Optical properties of ultrashort semiconducting single-walled carbon nanotube capsules down to sub-10 nm. J. Am. Chem. Soc. 130, 6551-6555, (2008).
  18. Sun, X., Liu, Z., Welsher, K., Robinson, J., Goodwin, A., Zaric, S. & Dai, H. Nano-graphene oxide for cellular imaging and drug delivery. Nano Research 1, 203-212, (2008).
  19. Goodwin, A. P., Tabakman, S. M., Welsher, K., Sherlock, S. P., Prencipe, G. & Dai, H. Phospholipid-Dextran with a Single Coupling Point: A Useful Amphiphile for Functionalization of Nanomaterials. J. Am. Chem. Soc. 131, 289-296, (2008).
  20. Li, X. L., Tu, X. M., Zaric, S., Welsher, K., Seo, W. S., Zhao, W. & Dai, H. J. Selective synthesis combined with chemical separation of single-walled carbon nanotubes for chirality selection. J. Am. Chem. Soc. 129, 15770-15771, (2007).
  21. Nakayama-Ratchford, N., Bangsaruntip, S., Sun, X., Welsher, K. & Dai, H. Noncovalent Functionalization of Carbon Nanotubes by Fluorescein-Polyethylene Glycol: Supramolecular Conjugates with pH-Dependent Absorbance and Fluorescence. J. Am. Chem. Soc. 129, 2448-2449, (2007).